ServicesFragment analysis
Fragmentanalysis.
Capillary genotyping in our Sanger core facility, from DNA fingerprinting to indel mutation analysis.
Run parameters
Example trace- AnalyserABI 3730XL genetic analyser
- DyesSelected for your panel
- Size standardSelected for your projectTell the team your expected fragment range before submitting.
- PolymerPOP-7
Read a trace
What comes back from a run.
Every peak is a labelled fragment, sized in base pairs against the LIZ500 ladder. Pick an example to see how alleles show up.
Application
Example sample
What you get back
Sample requirements
Confirm what your project needs.
Volumes, concentrations, dyes and size standards depend on the service and panel. Confirm them with the Sanger team before sending samples.
Primers
Confirm volume and concentrationrequirements vary with the number of reactions
Labelled primers
Protect from lightconfirm the current dye options for your panel
FA PCR products
Include dilution specificationsand protect labelled products from light
Genomic DNA
Confirm project requirementsbefore sending material for PCR amplification
Expected fragment range
Tell us before the runso the team can confirm the appropriate size standard
We discard samples and primers 2 months after your order is completed. To keep them, make arrangements with us before then. Ask us
Start from what you have
Tick these on the form and we take on the earlier steps.
- Courier collection
We collect your samples by courier.
- gDNA extraction
We accept plates with bacterial, fungal and yeast cultures.
- PCR from gDNA
Send samples and primers at the right concentrations, and we amplify.
- Primer synthesis
Give sequences 5′ to 3′ and confirm the currently available fluorescent labels with the team.
Animal profiles and parentage
Use the current ZooOmics catalogue and submission forms for animal DNA profiles, parentage and species-specific testing.
Browse ZooOmics testsHow to submit
Four steps to your sizes.
- 1
Download the form
The Sanger FA services form covers your details, the services you need, your samples and your primers.
Word form, 436 KB - 2
List samples and primers
Give each primer's marker name, dye, concentration, size range and annealing temperature.
Requirements - 3
Pack your samples
Print the sample page for the box. Triple packaging, labelled for its category, kept out of the light.
How to pack - 4
Send or book a collection
Courier your parcel to our Pretoria laboratory, or ask us to collect it.
Send or collect
Results usually arrive within 7 to 14 days of us receiving your samples, depending on project size, and are released once the order is paid.
Ask about yoursDesigning a marker panel?
Every fragment analysis project is different. Talk to the Sanger team about dyes, size ranges, multiplexing or a quote.